It is possible to get false negatives with samples collected in the early phases of infection - if such is the case, a sample should be collected again
To increase sensitivity, samples can be collected at 3 day intervals
IgG alone are not sufficient to diagnose acute Ebola Virus Disease because IgG persist from a previous infection even after the person has recovered and is no longer contagiousv
IgG/IgM cross-reaction with other pathogens is possible
New VHF strains or viruses may appear which could potentially not be easily detected