Contents

Teaching objectives
  1. Introduction to Molecular Biology
  2. 1. Structure of Desoxyribonucleic Acid (DNA)
    1. 1.1 Nucleotides
    2. 1.2 Phosphodiester bond
    3. 1.3 Double-stranded DNA
  3. 2. Properties of DNA
    1. 2.1 DNA is negatively charged
    2. 2.2 DNA absorbs ultra-violet light
    3. 2.3 Denatured DNA
    4. 2.4 Tm calculation
    5. 2.5 Renaturated DNA
    6. 2.6 Hybridization
  4. 3. DNA replication
    1. 3.1 Replication is semi-conservative
    2. 3.2 The replication fork
    3. 3.3 The chemistry of DNA replication
    4. 3.4 Steps of DNA replication
    5. 3.5 Continuous and discontinuous replication
    6. 3.6 Replisomes
  5. 4. Structure of the chromosomes
    1. 4.1 Definitions
    2. 4.2 The eukaryotic chromosome
    3. 4.3 The prokaryotic chromosome
  6. 5. The role of DNA
  7. Exercices